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Bioreactors are widely used to produce different therapeutics in the biopharmaceutical and regenerative medicine industries. Drug development relies on small multi-well plates shaken around an orbital diameter, while production-scale bioreactors are agitated by stirring. These different methods yield different fluid dynamics, making it difficult to scale lab findings to industry.
A team of researchers from University College London is starting to bridge this gap by applying analytical techniques for stirred bioreactors to the fluid dynamics of orbitally shaken bioreactors (OSBs). Combining vertical and horizontal measurements through particle image velocimetry, the group reconstructed a 3-D model of OSB flow and determined key features of the coherent structures inside OSBs. They publish their work this week in Physics of Fluids.
Work - Decomposition - Techniques - Modes - Oscillation
"In this work, we used two different decomposition techniques, which allowed us to identify dominant modes of oscillation of the flow inside in the reactor," said Andrea Ducci, an author on the paper. "The first pair of modes controls the free surface motion and therefore the aeration of cells, while the second pair is related to the bulk flow of the tank."
Shaken bioreactors offer low shear stresses and well-defined free surfaces of oxygen transfer, a gentle swirling that is vital for culturing mammalian cells. Proper orthogonal decomposition (POD), ranks modes by energy, while dynamic mode decomposition (DMD), orders them...
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