Despite great efforts in medicine and science, more than 400,000 people worldwide are still dying of malaria. The infectious disease is transmitted by the bite of mosquitoes infected with the malaria parasite Plasmodium. The genome of the parasite is relatively small with about 5,000 genes. In contrast to human cells, Plasmodium parasites only have a single copy of each individual gene. If one removes a gene from the entire genome of the parasite, this leads therefore directly to a change in the phenotype of the parasite. An international consortium led by Professors Volker Heussler from the Institute of Cell Biology (ICB) at the University of Bern and Oliver Billker from the Umeå University in Sweden and formerly at the Sanger Institute in Great Britain has taken advantage of this fact. The researchers have carried out a genome-wide gene deletion study on malaria parasites: They specifically removed over 1300 individual genes, observed the effects during the entire life cycle of the parasite and were thus able to identify many new targets in the pathogen.
The researchers used a malaria mouse model established at the Institute of Cell Biology at the University of Bern. Each of the 1300 parasite genes was replaced by an individual genetic code to analyze how the removal of the individual genes affects the parasite. The use of individual codes allows to study many parasites simultaneously and thus drastically shortens the time of their analysis. After three years of research, the international consortium succeeded in systematically screening the genome of the parasite in all life cycle stages. "The deletion screen carried out jointly with the Sanger Institute enabled us to identify hundreds of targets, particularly in the parasite's metabolism," said Rebecca Stanway from the ICB, one of the lead authors of this study.
To systematically analyze the large number of...
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