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Researchers at the Center for Genome Engineering, within the Institute for Basic Science (IBS, South Korea) have identified the mistake rate of DNA editing tools known as adenine base editors, which are based on CRISPR. Assessing the genome-wide target specificity of these innovative techniques is essential to harness their applications in clinics and biotechnology. The findings were published in Nature Biotechnology.
DNA's four bases are the alphabet used by our cells: adenine (A) pairs with thymine (T), cytosine (C) with guanine (G), making a unique combination of 3.2 billion letters. Since some genetic diseases are caused by a mutation of just one letter, some of the applications of CRISPR, a powerful gene engineering tool, deal with the correction of this single-letter difference. Examples of proteins that can be added to the CRISPR system to promote letter conversions are cytosine base editors (CBEs) for C-to-T conversions, and adenine base editors (ABEs) for A-to-G changes. The IBS team has been interested in studying ABE specificity, as it has not been known so far.
Team - Jin-Soo - Kim - Error - Rate
The team, led by Jin-Soo Kim, studied the error rate of recently developed ABE proteins, ABE7.10, in human cells. They pinpointed the positions on the human genome affected by ABE7.10 and scanned for errors beyond the target. To do that, they used an adapted version...
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