Study finds wide variation between human cell lines used for research

phys.org | 2/19/2019 | Staff
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More than 70 percent of researchers failed to reproduce another scientist's experiment, according to a survey conducted by Nature in 2016. If scientists can't reproduce each other's work, important discoveries may never leave the lab and have an impact on human health. One cause for this "reproducibility crisis" may lie in the significant differences in cell lines used by different laboratories, according to Yale researcher Yansheng Liu.

To explore the issue, Liu and a team of international scientists gathered samples of a human cancer cell line, known as HeLa cells, from more than a dozen laboratories of six countries worldwide. Established in the early 1950s, HeLa is the oldest and most commonly used human cell line. They cultured the different HeLa cells under uniform conditions and observed gene expression, growth rate, protein function, and other factors over time by using the state-of-the-art proteomic methods and other large-scale analysis. Additionally, the research team designed an experiment to study the response of the cell lines to Salmonella infection.

Research - Team - Variation

The research team found significant variation both between and...
(Excerpt) Read more at: phys.org
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