Unveiling the mechanism protecting replicated DNA from degradation

phys.org | 10/1/2018 | Staff
DanRules394 (Posted by) Level 3
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Researchers from Tokyo Metropolitan University and the FIRC Institute of Molecular Oncology (IFOM) in Italy have succeeded in depleting AND-1, a key protein for DNA replication, by using a recently developed conditional protein degradation system. They have gained unprecedented access to the underlying mechanism and determined that during DNA replication and cell proliferation in vertebrate cells, AND-1 has two different functions mediated by different domains of AND-1.

In order for living organisms to function, it is vital that all cells share the same DNA blueprint. This is made possible by the process of DNA replication, where the DNA is accurately copied and distributed before the cell multiplies. Replication underpins all biological inheritance, and is supported by a whole range of biochemical pathways designed to ensure that it occurs without error and at the right speed. Failure to do so can have catastrophic consequences, including cancer. Understanding the specific mechanisms behind this highly complex procedure is of the utmost importance.

AND-1/Ctf4 - Protein - Player - DNA - Replication

The AND-1/Ctf4 protein is a key player in DNA replication, and is found in a vast range of living organisms. Ctf4/AND-1 is essential in some organisms, but whether it is an essential gene for cell proliferation in vertebrates has not been shown experimentally. Moreover, how loss of AND-1 affects cell proliferation is not known.

In order to address this question, a team led by Dr. Dana Branzei from IFOM and Prof. Kouji Hirota from Tokyo Metropolitan University combined the DT40 cell, a type of avian cell that is particularly suited to genetic engineering, and the auxin-inducible degron (AID) system, a means of selective depletion of a target...
(Excerpt) Read more at: phys.org
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